基于纳米二氧化硅和“未保护”铂纳米簇的葡萄糖生物传感器。
Glucose biosensor based on nano-SiO2 and "unprotected" Pt nanoclusters.
作者信息
Yang Haipeng, Zhu Yongfa
机构信息
Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
出版信息
Biosens Bioelectron. 2007 Jun 15;22(12):2989-93. doi: 10.1016/j.bios.2006.12.019. Epub 2006 Dec 27.
The "unprotected" Pt nanoclusters (average size 2 nm) mixed with the nanoscale SiO(2) particles (average size 13 nm) were used as a glucose oxidase immobilization carrier to fabricate the amperometric glucose biosensor. The bioactivity of glucose oxidase (GOx) immobilized on the composite was maintained and the as-prepared biosensor demonstrated high sensitivity (3.85 microA mM(-1)) and good stability in glucose solution. The Pt-SiO(2) biosensor showed a detection limit of 1.5 microM with a linear range from 0.27 to 4.08 mM. In addition, the biosensor can be operated under wide pH range (pH 4.9-7.5) without great changes in its sensitivity. Cyclic voltammetry measurements showed a mixed controlled electrode reaction.
将“未受保护的”铂纳米团簇（平均尺寸2纳米）与纳米级二氧化硅颗粒（平均尺寸13纳米）混合，用作葡萄糖氧化酶固定载体，以制备电流型葡萄糖生物传感器。固定在复合材料上的葡萄糖氧化酶（GOx）的生物活性得以保持，所制备的生物传感器在葡萄糖溶液中表现出高灵敏度（3.85微安毫摩尔⁻¹）和良好的稳定性。铂-二氧化硅生物传感器的检测限为1.5微摩尔，线性范围为0.27至4.08毫摩尔。此外，该生物传感器可在较宽的pH范围（pH 4.9 - 7.5）下运行，其灵敏度变化不大。循环伏安法测量显示为混合控制电极反应。
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