Zou Yongjin, Xiang Cuili, Sun Li-Xian, Xu Fen
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Biosens Bioelectron. 2008 Feb 28;23(7):1010-6. doi: 10.1016/j.bios.2007.10.009. Epub 2007 Oct 25.
A novel amperometric biosensor, based on electrodeposition of platinum nanoparticles onto multi-walled carbon nanotube (MWNTs) and immobilizing enzyme with chitosan-SiO(2) sol-gel, is presented in this article. MWNTs were cast on the glass carbon (GC) substrate directly. An extra Nafion coating was used to eliminate common interferents such as acetaminophen and ascorbic acids. The morphologies and electrochemical performance of the modified electrodes have been investigated by scanning electron microscopy (SEM) and amperometric methods, respectively. The synergistic action of Pt and MWNTs and the biocompatibility of chitosan-SiO(2) sol-gel made the biosensor have excellent electrocatalytic activity and high stability. The resulting biosensor exhibits good response performance to glucose with a wide linear range from 1 microM to 23 mM and a low detection limit 1 microM. The biosensor also shows a short response time (within 5s), and a high sensitivity (58.9 microAm M(-1)cm(-2)). In addition, effects of pH value, applied potential, rotating rate, electrode construction and electroactive interferents on the amperometric response of the sensor were investigated and discussed in detail.
本文介绍了一种新型的安培生物传感器，该传感器基于将铂纳米颗粒电沉积到多壁碳纳米管（MWNTs）上，并使用壳聚糖 - SiO₂ 溶胶 - 凝胶固定酶。MWNTs 直接浇铸在玻碳（GC）基底上。使用额外的 Nafion 涂层来消除对乙酰氨基酚和抗坏血酸等常见干扰物。分别通过扫描电子显微镜（SEM）和安培法研究了修饰电极的形貌和电化学性能。Pt 和 MWNTs 的协同作用以及壳聚糖 - SiO₂ 溶胶 - 凝胶的生物相容性使生物传感器具有优异的电催化活性和高稳定性。所得生物传感器对葡萄糖表现出良好的响应性能，线性范围宽，从 1 μM 到 23 mM，检测限低至 1 μM。该生物传感器还具有短响应时间（5 秒内）和高灵敏度（58.9 μA mM⁻¹ cm⁻²）。此外，详细研究并讨论了 pH 值、施加电位、旋转速率、电极结构和电活性干扰物对传感器安培响应的影响。
