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[凝集反应和免疫酶分析法在检测D型葡萄球菌肠毒素中的应用]

[The use of the aggregate hemagglutination reaction and immunoenzyme analysis in determining staphylococcal enterotoxin type D].

作者信息

Beĭlbaeva M L, Naubet'iarov R B, Nurkina N M, Ezepchuk Iu V

出版信息

Lab Delo. 1991(7):61-4.

PMID:1724045
Abstract

Type D purified staphylococcal enterotoxin and anti-enterotoxic serum to it were prepared. IgG isolated from the anti-enterotoxic serum by the affinity chromatography method was used to improve the specificity and sensitivity of the aggregate hemagglutination test (AHAT) and enzyme immunoassay (EIA). The developed AHAT and EIA variants intended for the indication of type D staphylococcal enterotoxin are characterized by a high resolution ability and strict specificity. The suggested methods are simple and may be used for the rapid diagnosis of staphylococcal toxin infections.

摘要

制备了D型纯化葡萄球菌肠毒素及其抗肠毒素血清。通过亲和层析法从抗肠毒素血清中分离出的IgG用于提高凝集试验(AHAT)和酶免疫测定(EIA)的特异性和灵敏度。所开发的用于检测D型葡萄球菌肠毒素的AHAT和EIA变体具有高分辨率能力和严格的特异性。所建议的方法简单,可用于葡萄球菌毒素感染的快速诊断。

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