夜香树提取物的杀精活性。
Spermicidal activity of extract from Cestrum parqui.
作者信息
Souad Kammoun, Ali Saad, Mounir Ajina, Mounir Trabelsi Mohamed
机构信息
Laboratory of Histology and Embryology, Faculty of Medicine, 4002 Sousse, Tunisia.
出版信息
Contraception. 2007 Feb;75(2):152-6. doi: 10.1016/j.contraception.2006.10.006. Epub 2006 Dec 22.
OBJECTIVE
The leaf extract of Cestrum parqui was examined for its effects on sperm motility in vitro.
METHODS
Washed sperm were prepared by discontinuous buoyant density gradient centrifugation and incubated with varying concentrations of extract from C. parqui (40-250 microg/mL) at 37 degrees C and 5% CO(2). Sperm motility and viability were assessed at different intervals ranging from 5 to 240 min. Morphological changes in human ejaculated spermatozoa after exposure to the extract were evaluated under transmission electron microscope.
RESULTS
A dose- and time-dependent effect of this extract on sperm motility and viability was observed. The maximal spermicidal effect was observed with a 250-microg/mL dose of the extract. Transmission electron microscope revealed a significant damage to sperm membrane in both head and acrosomal membranes, notable swelling and disruption.
CONCLUSION
The present study indicates that this natural extract has potential spermicidal effect in vitro.
目的
研究夜香树叶提取物对体外精子活力的影响。
方法
通过不连续浮力密度梯度离心制备洗涤后的精子，并在37℃和5%二氧化碳条件下与不同浓度（40 - 250微克/毫升）的夜香树提取物孵育。在5至240分钟的不同时间间隔评估精子活力和存活率。在透射电子显微镜下评估暴露于提取物后人射精精子的形态变化。
结果
观察到该提取物对精子活力和存活率具有剂量和时间依赖性影响。提取物剂量为250微克/毫升时观察到最大杀精效果。透射电子显微镜显示精子头部和顶体膜的膜均有明显损伤，显著肿胀和破裂。
结论
本研究表明这种天然提取物在体外具有潜在的杀精作用。
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