Swerdlow Russell H
Department of Neurology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
J Neurosci Res. 2007 Nov 15;85(15):3416-28. doi: 10.1002/jnr.21167.
The cytoplasmic hybrid (cybrid) technique allows investigators to express selected mitochondrial DNA (mtDNA) sequences against fixed nuclear DNA (nDNA) backgrounds. Cybrids have been used to study the effects of known mtDNA mutations on mitochondrial biochemistry, mtDNA-nDNA inter-species compatibility, and mtDNA integrity in persons without mtDNA mutations defined previously. This review discusses events leading up to creation of the cybrid technique, as well as data obtained via application of the cybrid strategies listed above. Although interpreting cybrid data requires awareness of technique limitations, valuable insights into mtDNA genotype-functional phenotype relationships are suggested.
细胞质杂种（cybrid）技术使研究人员能够在固定的核DNA（nDNA）背景下表达选定的线粒体DNA（mtDNA）序列。Cybrids已被用于研究已知mtDNA突变对线粒体生物化学、mtDNA-nDNA种间兼容性以及先前未定义mtDNA突变的个体中mtDNA完整性的影响。本综述讨论了导致cybrid技术产生的事件，以及通过应用上述cybrid策略获得的数据。尽管解释cybrid数据需要了解技术局限性，但仍能从中获得有关mtDNA基因型与功能表型关系的宝贵见解。
