Gamez Gerardo, Ray Steven J, Andrade Francisco J, Webb Michael R, Hieftje Gary M
Department of Chemistry, Indiana University Bloomington, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, USA.
Anal Chem. 2007 Feb 15;79(4):1317-26. doi: 10.1021/ac061361l. Epub 2007 Jan 23.
Glow discharge optical emission spectrometry has cemented itself as an important surface elemental analysis technique in part because of its superb depth resolution (on the order of single nanometers). However, very few studies have explored the ability of the glow discharge to provide laterally resolved elemental information. In the present study, an end-on-viewed pulsed radio frequency glow discharge is coupled to a monochromatic imaging spectrometer to provide lateral surface imaging. The performance of the technique is demonstrated with etched copper circuits on fiber-glass substrates, and it is shown how several operating parameters including pressure, pulsed mode operation, and time-resolved detection affect the lateral surface resolution. In addition, because a pulsed radio frequency glow discharge offers elemental information on nonconducting samples, the technique is applied to the three-dimensional elemental analysis of proteins on blotting substrates. Several alternative sample types are also examined, including photographic film and glass.
辉光放电光发射光谱法已成为一种重要的表面元素分析技术，部分原因在于其出色的深度分辨率（可达单纳米量级）。然而，很少有研究探讨辉光放电提供横向分辨元素信息的能力。在本研究中，将端视脉冲射频辉光放电与单色成像光谱仪相结合，以提供横向表面成像。该技术的性能通过玻璃纤维基板上的蚀刻铜电路得到了证明，并且展示了包括压力、脉冲模式操作和时间分辨检测在内的几个操作参数如何影响横向表面分辨率。此外，由于脉冲射频辉光放电可提供非导电样品的元素信息，该技术被应用于印迹基板上蛋白质的三维元素分析。还研究了几种其他类型的样品，包括摄影胶片和玻璃。
