Persson H
University of Göteborg, Department of Anatomy, Sweden.
Neurochem Res. 1991 Oct;16(10):1113-20. doi: 10.1007/BF00966588.
The presence of degradation products of the myelin/oligodendrocyte glycoprotein (MOG) and a new myelin/oligodendrocyte associated protein, FD1, defined by a monoclonal antibody was established in a subfraction (the floating fraction, or FF) of adult rabbit CNS. The histochemical distribution of FD1 was determined by indirect immunofluorescence using conventional and confocal microscopy. FD1 was found to be present in oligodendrocytes, and at the outer rim of CNS myelin sheaths. Strong antibody reactivity was noted at nodes of Ranvier, as well as in regions with a high nodal density. No staining of compact myelin was seen. In the PNS, inner and outer cytoplasmic compartments of the Schwann cells as well as their cell bodies were stained, with no staining of compact myelin. The FF has previously been shown to be highly enriched in Marchi-positive bodies. These structures are situated paranodally in the CNS of myelinated nerve fibers, and their presence has been interpreted as reflections of myelin breakdown and turnover occurring in association with myelin sheath segments situated close to nodes at Ranvier in adult, normal vertebrate CNS. The present findings extend previous observations of partially degraded myelin-associated proteins in the FF, and give further results indicating that Marchi-positive bodies are aspects of intermediate stages in myelin catabolism.
通过单克隆抗体鉴定的髓鞘/少突胶质细胞糖蛋白（MOG）降解产物和一种新的髓鞘/少突胶质细胞相关蛋白FD1在成年兔中枢神经系统的一个亚组分（漂浮组分，即FF）中被证实存在。使用传统显微镜和共聚焦显微镜通过间接免疫荧光法确定了FD1的组织化学分布。发现FD1存在于少突胶质细胞以及中枢神经系统髓鞘的外缘。在郎飞结处以及结密度高的区域观察到强抗体反应性。未见到致密髓鞘的染色。在周围神经系统中，施万细胞的内、外细胞质区室及其细胞体被染色，致密髓鞘未染色。先前已表明FF中富含Marchi阳性小体。这些结构位于有髓神经纤维中枢神经系统的结旁，其存在被解释为与成年正常脊椎动物中枢神经系统中靠近郎飞结的髓鞘节段相关的髓鞘分解和更新的反映。目前的研究结果扩展了先前对FF中部分降解的髓鞘相关蛋白的观察，并给出了进一步的结果，表明Marchi阳性小体是髓鞘分解代谢中间阶段的表现。
