生菜（Lactuca sativa L.）遗传图谱，包含限制片段长度多态性、同工酶、抗病性和形态学标记。
A Genetic Map of Lettuce (Lactuca sativa L.) with Restriction Fragment Length Polymorphism, Isozyme, Disease Resistance and Morphological Markers.
机构信息
Department of Vegetable Crops, University of California, Davis, California 95616.
出版信息
Genetics. 1987 Jun;116(2):331-7. doi: 10.1093/genetics/116.2.331.
A detailed linkage map of lettuce was constructed using 53 genetic markers including 41 restriction fragment length polymorphism (RFLP) loci, five downy mildew resistance genes, four isozyme loci and three morphological markers. The genetic markers were distributed into nine linkage groups and cover 404 cM which may be 25-30% of the lettuce genome. The majority (31 of 34) of the RFLP probes detected single segregating loci, although seven of these may have been homologous to further monomorphic loci. When several loci were detected by a single probe, the loci were generally linked, suggesting tandem duplications. One probe, however, detected loci in three linkage groups suggesting translocations. The five downy mildew resistance genes (Dm1, Dm3, Dm4, Dm5/8 and Dm13), segregating in the Calmar x Kordaat cross, represented each of the four resistance gene linkage groups. Dm5/8 is flanked by two cDNA loci, each located 10 cM away. These flanking markers will be used to study the source of variation in downy mildew genes and are also part our strategy to clone resistance genes.
利用包括 41 个限制片段长度多态性（RFLP）标记、5 个霜霉病抗性基因、4 个同工酶标记和 3 个形态标记在内的 53 个遗传标记，构建了生菜的详细连锁图谱。这些遗传标记分布在 9 个连锁群中，覆盖 404cM，可能占生菜基因组的 25-30%。虽然其中的 7 个可能与进一步的单态性基因座同源，但大多数（31 个中的 34 个）RFLP 探针检测到单分离基因座。当单个探针检测到多个基因座时，这些基因座通常是连锁的，表明串联重复。然而，有一个探针检测到三个连锁群中的基因座，表明发生了易位。在 Calmar x Kordaat 杂交中分离的 5 个霜霉病抗性基因（Dm1、Dm3、Dm4、Dm5/8 和 Dm13）分别代表四个抗性基因连锁群中的每一个。Dm5/8 被两个 cDNA 基因座包围，每个基因座距离 10cM。这些侧翼标记将用于研究霜霉病基因的变异来源，也是我们克隆抗性基因策略的一部分。
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