基于 RFLP、RAPD 和同工酶标记的日本柳杉（Cryptomeria japonica）连锁图谱。

A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci.

机构信息

Forestry and Forest Products Research Institute, Tsukuba Norin Kenkyu Danchi-nai, POB. 16, 305, Ibaraki, Japan.

出版信息

Theor Appl Genet. 1995 May;90(6):835-40. doi: 10.1007/BF00222019.

DOI:10.1007/BF00222019

Abstract

A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.

摘要

利用为心材颜色遗传分析而准备的三代系谱，基于限制片段长度多态性（RFLP）、随机扩增多态性 DNA（RAPD）和同工酶位点构建了日本落叶松的连锁图谱。总共分离出 128 个 RFLP（123 个 cDNA 和 5 个基因组探针）、33 个 RAPD、2 个同工酶和 1 个形态（矮化）位点，在 73 个后代中分离。在 164 个分离的位点中，有 145 个位点分布在 20 个连锁群中。其中，91 个具有明确图谱位置的位点被分配到 13 个连锁群中，总长度为 887.3cM。在 6 个连锁群中观察到标记的聚类与偏分离。在四个聚类中，仅从一个亲本中发现了减少纯合子数量的偏分离。