Gräslund A, Ehrenberg A, Rupprecht A, Ström G, Crespi H
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Oct;28(4):313-23. doi: 10.1080/09553007514551101.
The free radicals induced by gamma-irradiation at 77 K in non-deuterated and fully deuterated oriented samples of moist algal DNA have been studied by electron paramagnetic resonance (e.p.r.). The e.p.r. spectra from the non-deuterated sample were found to be similar to those from calf-thymus DNA studied previously. Numerical spectra simulations were performed based on the previously proposed interpretation of the DNA spectra at 77 K in terms of a mixture of two spectral components arising from anionic and cationic base radicals (probably on thymine and guanine, respectively). The simulations were found to account satisfactorily for the main characteristics of the e.p.r. spectra from both the non-deuterated and the fully deuterated algal DNA sample.
通过电子顺磁共振(e.p.r.)研究了在77K下,γ射线辐照非氘代和全氘代取向的湿藻DNA样品所产生的自由基。发现非氘代样品的电子顺磁共振谱与先前研究的小牛胸腺DNA的谱相似。基于先前提出的对77K时DNA谱的解释,即由阴离子和阳离子碱基自由基(可能分别位于胸腺嘧啶和鸟嘌呤上)产生的两种光谱成分的混合物,进行了数值光谱模拟。结果发现,该模拟能够令人满意地解释非氘代和全氘代藻DNA样品的电子顺磁共振谱的主要特征。