Suppr超能文献

转基因番茄中转座激活子的性传播。

Sexual transmission of transposed activator elements in transgenic tomatoes.

机构信息

Department of Vegetable Crops, University of California, Davis, California 95616.

出版信息

Genetics. 1989 Sep;123(1):181-9. doi: 10.1093/genetics/123.1.181.

Abstract

The transmission of transposed Ac elements in progeny derived by self-pollination of ten transformed tomato plants has been examined by Southern hybridization analysis. We show that six of these primary transformants have transmitted a transposed Ac to at least one progeny. One of the families was segregating for at least two different insertion events. In five of ten families, progeny were detected that contained a transposed Ac but no donor T-DNA sequences, indicating that a recombination event occurred between the original and new Ac insertion site. Somatic transposition of Ac as late as the R2 generation is evidenced. One family contained an empty donor site fragment but Ac was not detected in either the parent or progeny, indicating Ac was lost in this population early in regeneration. While four of ten families were segregating for aberrant phenotypes, there was no evidence that the mutated gene was linked to a transposed Ac.

摘要

通过自交十株转化番茄植株的后代,我们通过 Southern 杂交分析研究了转座 Ac 元件的传递。结果表明,这六个初级转化体至少将一个 Ac 转座到一个后代中。其中一个家系至少分离出两个不同的插入事件。在十个家系中的五个中,检测到含有转座 Ac 但没有供体 T-DNA 序列的后代,这表明在原始和新 Ac 插入位点之间发生了重组事件。证明了 Ac 的体细胞转座在 R2 代已经发生。一个家系含有一个空的供体位点片段,但在亲本或后代中均未检测到 Ac,这表明在再生早期该群体中 Ac 丢失。虽然十个家系中有四个表现出异常表型,但没有证据表明突变基因与转座 Ac 相关。

相似文献

1
Sexual transmission of transposed activator elements in transgenic tomatoes.
Genetics. 1989 Sep;123(1):181-9. doi: 10.1093/genetics/123.1.181.
3
Use of Ac as an insertional mutagen in Arabidopsis.
Plant J. 1996 Jun;9(6):935-45. doi: 10.1046/j.1365-313x.1996.9060935.x.
4
Independent transposition of multiple Ac elements in the same transgenic tomato cell.
Plant Mol Biol. 1992 Apr;18(6):1065-72. doi: 10.1007/BF00047709.
5
Transposition of the maize controlling element "Activator" in tobacco.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844-8. doi: 10.1073/pnas.83.13.4844.
9
Transposition of the maize activator element in transgenic rice plants.
Nucleic Acids Res. 1991 Feb 11;19(3):617-22. doi: 10.1093/nar/19.3.617.
10

引用本文的文献

3
Selection for enhanced germinal excision of Ac in transgenic Arabidopsis thaliana.
Theor Appl Genet. 1993 Sep;86(8):919-26. doi: 10.1007/BF00211042.
4
Precise marker excision system using an animal-derived piggyBac transposon in plants.
Plant J. 2014 Feb;77(3):454-63. doi: 10.1111/tpj.12367. Epub 2013 Dec 9.
5
The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants.
Plant Mol Biol. 2013 Jan;81(1-2):149-60. doi: 10.1007/s11103-012-9988-9. Epub 2012 Nov 21.
6
A hyperactive transposase of the maize transposable element activator (Ac).
Genetics. 2012 Jul;191(3):747-56. doi: 10.1534/genetics.112.139642. Epub 2012 May 4.
7
Selection of marker-free transgenic plants using the isopentenyl transferase gene.
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2117-21. doi: 10.1073/pnas.94.6.2117.
8
Infrequent transposition of Ac in lettuce, Lactuca sativa.
Plant Mol Biol. 1993 Aug;22(5):793-805. doi: 10.1007/BF00027366.
9
Ac-induced instability at the Xanthophyllic locus of tomato.
Genetics. 1993 Jul;134(3):931-42. doi: 10.1093/genetics/134.3.931.

本文引用的文献

2
Transposition of the maize controlling element "Activator" in tobacco.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844-8. doi: 10.1073/pnas.83.13.4844.
3
Phenotypic assay for excision of the maize controlling element Ac in tobacco.
EMBO J. 1987 Jun;6(6):1547-54. doi: 10.1002/j.1460-2075.1987.tb02399.x.
5
Plant transposable elements generate the DNA sequence diversity needed in evolution.
EMBO J. 1985 Mar;4(3):591-7. doi: 10.1002/j.1460-2075.1985.tb03671.x.
6
Chromosome organization and genic expression.
Cold Spring Harb Symp Quant Biol. 1951;16:13-47. doi: 10.1101/sqb.1951.016.01.004.
7
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
8
Classical and molecular genetics of tomato: highlights and perspectives.
Annu Rev Genet. 1988;22:281-300. doi: 10.1146/annurev.ge.22.120188.001433.
9
Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification.
EMBO J. 1987 Feb;6(2):295-302. doi: 10.1002/j.1460-2075.1987.tb04753.x.
10

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验