Department of Vegetable Crops, University of California, Davis, California 95616.
Genetics. 1989 Sep;123(1):181-9. doi: 10.1093/genetics/123.1.181.
The transmission of transposed Ac elements in progeny derived by self-pollination of ten transformed tomato plants has been examined by Southern hybridization analysis. We show that six of these primary transformants have transmitted a transposed Ac to at least one progeny. One of the families was segregating for at least two different insertion events. In five of ten families, progeny were detected that contained a transposed Ac but no donor T-DNA sequences, indicating that a recombination event occurred between the original and new Ac insertion site. Somatic transposition of Ac as late as the R2 generation is evidenced. One family contained an empty donor site fragment but Ac was not detected in either the parent or progeny, indicating Ac was lost in this population early in regeneration. While four of ten families were segregating for aberrant phenotypes, there was no evidence that the mutated gene was linked to a transposed Ac.
通过自交十株转化番茄植株的后代,我们通过 Southern 杂交分析研究了转座 Ac 元件的传递。结果表明,这六个初级转化体至少将一个 Ac 转座到一个后代中。其中一个家系至少分离出两个不同的插入事件。在十个家系中的五个中,检测到含有转座 Ac 但没有供体 T-DNA 序列的后代,这表明在原始和新 Ac 插入位点之间发生了重组事件。证明了 Ac 的体细胞转座在 R2 代已经发生。一个家系含有一个空的供体位点片段,但在亲本或后代中均未检测到 Ac,这表明在再生早期该群体中 Ac 丢失。虽然十个家系中有四个表现出异常表型,但没有证据表明突变基因与转座 Ac 相关。