Andersson S E, Le Greves P
Department of Physiology and Medical Biophysics, University of Uppsala, Sweden.
Eur J Pharmacol. 1991 Dec 17;209(3):175-83. doi: 10.1016/0014-2999(91)90167-o.
The effects of ruthenium red, an inorganic dye with known capsaicin antagonist properties, was investigated in the rabbit eye. At a dose of 0.24 nmol ruthenium red inhibited the inflammatory effects of capsaicin (1 or 8 nmol). Unexpectedly, when the dye was injected in doses ranging from 0.24 to 7.4 nmol, it caused an inflammatory response with constriction of the pupil (miosis) and a breakdown of the blood-aqueous barrier, leading to a rise intraocular pressure. Tetrodotoxin (30 nmol) inhibited the ruthenium red-induced rise in intraocular pressure but had less effect on the miotic response. The tachykinin antagonist spantide inhibited the miosis but had no effect on the rise in intraocular pressure. Ruthenium red induced an increase in substance P-like immunoreactivity and calcitonin gene-related peptide-like immunoreactivity in the aqueous humor. These levels were positively correlated with the rise in aqueous humor protein concentration. The ruthenium red-induced miosis and, to a less extent, the rise in intraocular pressure were inhibited by the Ca2+ channel-blocking agent omega-conotoxin GVIA (CTX), indicating a partial dependence on an influx of extracellular Ca2+. CTX also attenuated the miotic effect of capsaicin but had no effect on the capsaicin-induced rise in intraocular pressure. It is concluded that, in the rabbit eye, ruthenium red induces a neurogenic inflammatory response besides its capsaicin antagonist effects.
在兔眼中研究了钌红（一种具有已知辣椒素拮抗剂特性的无机染料）的作用。在剂量为0.24 nmol时，钌红抑制了辣椒素（1或8 nmol）的炎症作用。出乎意料的是，当以0.24至7.4 nmol的剂量注射该染料时，它会引起炎症反应，伴有瞳孔收缩（瞳孔缩小）和血-房水屏障破坏，导致眼压升高。河豚毒素（30 nmol）抑制了钌红诱导的眼压升高，但对瞳孔缩小反应的影响较小。速激肽拮抗剂spantide抑制了瞳孔缩小，但对眼压升高没有影响。钌红诱导房水中P物质样免疫反应性和降钙素基因相关肽样免疫反应性增加。这些水平与房水蛋白浓度的升高呈正相关。钌红诱导的瞳孔缩小以及在较小程度上的眼压升高被Ca2+通道阻滞剂ω-芋螺毒素GVIA（CTX）抑制，表明部分依赖于细胞外Ca2+的流入。CTX也减弱了辣椒素的瞳孔缩小作用，但对辣椒素诱导的眼压升高没有影响。得出的结论是，在兔眼中，钌红除了具有辣椒素拮抗剂作用外，还诱导神经源性炎症反应。
