Murphy M, Chen J N, George D L
Department of Human Genetics, University of Pennsylvania, Philadelphia.
J Neurosci Res. 1991 Nov;30(3):475-83. doi: 10.1002/jnr.490300304.
Human leptomeningeal cells grown in culture were immortalized via transfection with an SV40 large T antigen gene construct under the control of the Rous sarcoma virus promoter. This cell line, designated LTAg2B, maintained the polygonal morphology characteristic of primary cultures, and stained positively in early passage for cytokeratin, a specific marker for arachnoid cells of the leptomeninges. Additional immunofluorescent staining revealed that these cells express vimentin and desmoplakin as well; these antigens have been found together only in normal arachnoid tissue and in meningiomas, which are the neoplastic derivatives of leptomeningeal cells. Significantly, LTAg2B cells demonstrate a greatly increased life span and growth rate relative to primary cultures. The establishment of this cell line should thus facilitate studies on the cellular and molecular biology of leptomeningeal cells, as well as elucidate their roles in certain pathological situations involving the leptomeninges, such as meningitis and meningioma tumor formation.
通过用劳斯肉瘤病毒启动子控制下的SV40大T抗原基因构建体转染，在培养中生长的人软脑膜细胞被永生化。这个细胞系命名为LTAg2B，保持了原代培养的多边形形态特征，并且在早期传代时细胞角蛋白染色呈阳性，细胞角蛋白是软脑膜蛛网膜细胞的特异性标志物。额外的免疫荧光染色显示这些细胞也表达波形蛋白和桥粒斑蛋白；这些抗原仅在正常蛛网膜组织和脑膜瘤中同时被发现，脑膜瘤是软脑膜细胞的肿瘤衍生物。值得注意的是，相对于原代培养，LTAg2B细胞显示出大大延长的寿命和生长速率。因此，这个细胞系的建立应该有助于对软脑膜细胞的细胞和分子生物学的研究，以及阐明它们在涉及软脑膜的某些病理情况中的作用，如脑膜炎和脑膜瘤肿瘤形成。
