玉米淀粉支链延伸基因座的遗传精细结构分析
Genetic Fine Structure Analysis of the AMYLOSE-EXTENDER Locus in ZEA MAYS L.
机构信息
Department of Horticulture, The Pennsylvania State University, University Park, Pa. 16802.
出版信息
Genetics. 1972 Apr;70(4):611-9. doi: 10.1093/genetics/70.4.611.
Abstract
On the basis of interallelic recombination frequencies measured in diallelic crosses of the 5 amylose-extender alleles in maize, ae, B1, B3, M2 and i1, it was possible to construct a unique linear genetic map ordering all 5 alleles within the locus. The reciprocal diallelic crosses each gave comparable frequency estimates. The relative order is ae, i1, B3, B1, M2 or the reverse. Even though F1 endosperms resulting from all possible diallelic crosses were phenotypically mutant, therefore non-complementing, no decision as to whether or not these alleles exhibit functional complementation should be made without biochemical characterization of the starches from the various heteroallelic genotypes.
摘要
基于在玉米 5 个直链淀粉扩展等位基因的双等位基因杂交中测量的等位基因间重组频率，ae、B1、B3、M2 和 i1，可以构建一个独特的线性遗传图谱，将该基因座内的所有 5 个等位基因进行排序。相互的双等位基因杂交都给出了可比的频率估计值。相对顺序是 ae、i1、B3、B1、M2 或相反。尽管来自所有可能的双等位基因杂交的 F1 胚乳在表型上都是突变体，因此不互补，但在对各种杂合基因型的淀粉进行生化特性分析之前，不应就这些等位基因是否表现出功能互补做出决定。
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