Zoology Department, University of Illinois, Urbana, Illinois 61801.
Genetics. 1973 Aug;74(4):595-603. doi: 10.1093/genetics/74.4.595.
The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.
我们通过淀粉凝胶电泳技术,对共生 1 型的梨形四膜虫中天冬氨酸转氨酶、NADP 苹果酸脱氢酶、NADP 异柠檬酸脱氢酶和四唑氧化酶的同工酶模式进行了检测。我们通过一个繁殖计划研究了控制这些酶的等位基因的遗传。每个酶基因座都表现出与该生物中其他基因座一样的营养体分离现象。对天冬氨酸转氨酶基因座的分离过程进行详细分析表明,杂合体稳定为纯合子的速度与之前报道的其他基因座的速度基本相同。