梨形四膜虫的异柠檬酸脱氢酶

Isocitrate dehydrogenase of Tetrahymena pyriformis.

作者信息

Vidal P, Machado A

出版信息

Mol Cell Biochem. 1977 Oct 7;17(3):151-6. doi: 10.1007/BF01730835.

DOI:10.1007/BF01730835

PMID:22034

Abstract

We have studied the isocitrate dehydrogenase of Tetrahymena pyriformis. This enzyme is able to utilize both NAD and NADP, but kinetic studies suggest that the enzymatic activity with NAD is not of physiological signifance. Some of the factors that might regualte the NADP-dependent isocitrate dehydrogenase were also studied. This enzyme has an absolute requirement for divalent cations; Mg,+ and Mn2+ will serve as cofactors but the latter is more effective than the former. It is known that this enzyme is subject to a concerted inhibition by oxaloacetate and glyoxylate. Either glyoxylate or oxaloacetate alone also are capable of inhibiting the enzyme although higher concentrations are required. We have found concerted inhibition also for the NAD-dependent isocitrate dehydrogenase from rat liver and yeast. The activity of the Tetrahymena pyriformis enzyme is inhibited by NADPH. This inhibition is competitive with NADP. The Ki and Km values are, respectively, 20 micrometers and 18 micrometers.

摘要

我们研究了梨形四膜虫的异柠檬酸脱氢酶。这种酶能够利用NAD和NADP，但动力学研究表明，NAD的酶活性没有生理意义。我们还研究了一些可能调节依赖NADP的异柠檬酸脱氢酶的因素。这种酶对二价阳离子有绝对需求；Mg²⁺和Mn²⁺可作为辅因子，但后者比前者更有效。已知这种酶受到草酰乙酸和乙醛酸的协同抑制。单独的乙醛酸或草酰乙酸也能够抑制该酶，尽管需要更高的浓度。我们还发现大鼠肝脏和酵母中依赖NAD的异柠檬酸脱氢酶也存在协同抑制。梨形四膜虫的这种酶的活性受到NADPH的抑制。这种抑制与NADP竞争。Ki和Km值分别为20微摩尔和18微摩尔。

相似文献

1

Isocitrate dehydrogenase of Tetrahymena pyriformis.梨形四膜虫的异柠檬酸脱氢酶

Mol Cell Biochem. 1977 Oct 7;17(3):151-6. doi: 10.1007/BF01730835.

2

NADPH/NADP+ ratio: regulatory implications in yeast glyoxylic acid cycle.NADPH/NADP⁺ 比值：对酵母乙醛酸循环的调控意义

Mol Cell Biochem. 1983;51(2):123-7. doi: 10.1007/BF00230397.

3

[Regulation of the glyoxylic cycle: effect of the NADPH/NADP ratio].[乙醛酸循环的调节：NADPH/NADP 比值的影响]

Rev Esp Fisiol. 1982;38 Suppl:141-6.

4

Evidence suggesting that the NADPH/NADP ratio modulates the splitting of the isocitrate flux between the glyoxylic and tricarboxylic acid cycles, in Escherichia coli.有证据表明，在大肠杆菌中，NADPH/NADP比率调节乙醛酸循环和三羧酸循环之间异柠檬酸通量的分配。

FEBS Lett. 1979 Sep 15;105(2):333-6. doi: 10.1016/0014-5793(79)80642-0.

5

Modification of NAD-dependent isocitrate dehydrogenase by the 2',3'-dialdehyde derivatives of NAD, NADH, NADP, and NADPH.NAD、NADH、NADP和NADPH的2',3'-二醛衍生物对NAD依赖的异柠檬酸脱氢酶的修饰作用。

Arch Biochem Biophys. 1988 Aug 1;264(2):665-77. doi: 10.1016/0003-9861(88)90333-5.

6

Regulation of isocitrate metabolism in peroxisomes in Tetrahymena pyriformis.梨形四膜虫过氧化物酶体中异柠檬酸代谢的调控

Arch Biochem Biophys. 1972 Oct;152(2):463-71. doi: 10.1016/0003-9861(72)90240-8.

7

Variation in glyoxylate bypass inducibility among strains of Tetrahymena pyriformis.梨形四膜虫菌株间乙醛酸旁路诱导性的差异。

J Protozool. 1973 Aug;20(3):467-70. doi: 10.1111/j.1550-7408.1973.tb00928.x.

8

Inhibition of bovine heart NAD-specific isocitrate dehydrogenase by reduced pyridine nucleotides: modulation of inhibition by ADP, NAD+, Ca2+, citrate, and isocitrate.还原型吡啶核苷酸对牛心NAD特异性异柠檬酸脱氢酶的抑制作用：ADP、NAD⁺、Ca²⁺、柠檬酸和异柠檬酸对抑制作用的调节

Biochemistry. 1984 Jun 5;23(12):2773-8. doi: 10.1021/bi00307a037.

9

Kinetic mechanism of Escherichia coli isocitrate dehydrogenase and its inhibition by glyoxylate and oxaloacetate.大肠杆菌异柠檬酸脱氢酶的动力学机制及其受乙醛酸和草酰乙酸的抑制作用

Biochem J. 1986 Mar 1;234(2):317-23. doi: 10.1042/bj2340317.

10

Electrophoretic characterization of classical Tetrahymena pyriformis strains.梨形四膜虫经典菌株的电泳特征分析。

J Protozool. 1973 Nov;20(5):693-700. doi: 10.1111/j.1550-7408.1973.tb03601.x.

引用本文的文献

1

NADPH/NADP+ ratio: regulatory implications in yeast glyoxylic acid cycle.NADPH/NADP⁺ 比值：对酵母乙醛酸循环的调控意义

Mol Cell Biochem. 1983;51(2):123-7. doi: 10.1007/BF00230397.

本文引用的文献

1

AMINO GROUP FORMATION AND GLUTAMATE SYNTHESIS IN STREPTOCOCCUS BOVIS.牛链球菌中的氨基形成与谷氨酸合成

J Bacteriol. 1964 Oct;88(4):1038-44. doi: 10.1128/jb.88.4.1038-1044.1964.

2

Control of the citric acid cycle by glyoxylate. 2. Mechanism of the inhibition of respiration in liver and kidney particles.乙醛酸对柠檬酸循环的调控。2. 肝脏和肾脏微粒体呼吸抑制机制。

Biochem J. 1962 Dec;85(3):593-600. doi: 10.1042/bj0850593.

3

Control of the etric acid cycle by glyoxylate. I. A new inhibitor of aconitase formed by the condensation of glyoxylate with oxaloacetate.乙醛酸对柠檬酸循环的调控。I. 乙醛酸与草酰乙酸缩合形成的乌头酸酶新抑制剂。

Biochem J. 1962 Dec;85(3):588-93. doi: 10.1042/bj0850588.

4

Levels of pyridine nucleotides and DPNH oxidase activity in growing and stationary protozoan cultures.生长和静止期原生动物培养物中吡啶核苷酸水平及二磷酸吡啶核苷酸氧化酶活性

Biochim Biophys Acta. 1962 Dec 17;65:411-8. doi: 10.1016/0006-3002(62)90442-0.

5

Induction of respiratory deficiency in yeast by manganese, copper, cobalt and nickel.锰、铜、钴和镍对酵母呼吸缺陷的诱导作用。

Nature. 1958 Aug 16;182(4633):446-8. doi: 10.1038/182446a0.

6

[The enzymatic properties of isocitric dehydrogenase].[异柠檬酸脱氢酶的酶学性质]

J Biol Chem. 1957 Jun;226(2):977-91.

7

Partial purification and characterization of the isocitric dehydrogenase from Trypanosoma cruzi.克氏锥虫异柠檬酸脱氢酶的部分纯化及特性分析

Biochim Biophys Acta. 1956 Jul;21(1):117-26. doi: 10.1016/0006-3002(56)90101-9.

8

Studies on the metabolism of the Protozoa. III. Metabolism of the ciliate Tetrahymena pyriformis (Glaucoma piriformis).原生动物代谢的研究。III. 梨形四膜虫（梨形青光眼虫）的代谢

Biochem J. 1952 Nov;52(3):483-92. doi: 10.1042/bj0520483.

9

Glyconeogenesis in Tetrahymena pyriformis. Relationship of enzyme adaptation to the carbon pathway.梨形四膜虫中的糖异生作用。酶适应性与碳代谢途径的关系。

J Biol Chem. 1967 Sep 25;242(18):4060-6.

10

Purification and reversible inactivation of the isocitrate dehydrogenase from an obligate halophile.来自专性嗜盐菌的异柠檬酸脱氢酶的纯化及可逆失活

J Bacteriol. 1969 Jul;99(1):161-8. doi: 10.1128/jb.99.1.161-168.1969.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。