Departamento de Bioquímica, E. T. S. Ingenieros Agrónomos, Madrid-3, Spain.
Genetics. 1978 May;89(1):147-56. doi: 10.1093/genetics/89.1.147.
Two hydrophobic endosperm proteins, designated CM3 and CM3', have been purified from appropriate cultivars of tetraploid wheat (T. turgidum) and characterized. They are inherited as though encoded by alleles at a single locus, designated Cm3a and Cm3b, respectively. The net amount of protein molecules has been measured for each of the alleles at one, two and three doses. The amount of CM3' is 50%-65% of that found for CM3. For both, there is a linear gene dosage response. These effects were observed not only in the parental material and the reciprocal F(1) generations, but also in the segregating F(2) generation, indicating that the quantitative difference depends on differences in the structural gene or is controlled by regulatory or modifier gene(s) linked to it.
已从适当的四倍体小麦(T. turgidum)品种中纯化并鉴定出两种疏水性胚乳蛋白,分别命名为 CM3 和 CM3'。它们作为单一位点的等位基因遗传,分别命名为 Cm3a 和 Cm3b。已测量每个等位基因在一个、两个和三个剂量下的蛋白质分子的净含量。CM3'的含量为 CM3 的 50%-65%。对于两者,都存在线性基因剂量响应。这些效应不仅在亲本材料和相互的 F1 代中观察到,而且在分离的 F2 代中也观察到,表明这种定量差异取决于结构基因的差异,或者受与其相连的调节或修饰基因控制。