等位基因间在玉米 sh 基因座的酶水平上的互补作用。
Interallelic Complementation at the sh Locus in Maize at the Enzyme Level.
机构信息
Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706.
出版信息
Genetics. 1979 Feb;91(2):317-25. doi: 10.1093/genetics/91.2.317.
Abstract
EMS-induced sh mutants and their heterozygotes were examined for the enzyme, sucrose synthetase, which has previously been shown to be coded by the Sh locus. Complementing heterozygotes have a wild-type phenotype, but show no hybrid protein band after starch gel electrophoresis. The existence of a heteromeric complex, however, is inferred from the two-fold elevation in sucrose cleavage activity in the complementing heterozygotes as compared to the mutant homozygotes. The observations on complementation described here are unique, as the elevation in the activity of this reversible enzyme is noticed only in one direction (viz, sucrose cleavage) of the reaction and not the other (sucrose synthesis).
摘要
对 EMS 诱导的 sh 突变体及其杂合体进行了蔗糖合酶（先前已表明该酶由 Sh 基因座编码）的检测。互补杂合体表现出野生型表型，但在淀粉凝胶电泳后没有显示出杂合蛋白带。然而，从互补杂合体中蔗糖裂解活性比突变纯合体提高了一倍这一事实，可以推断出存在异源寡聚复合物。这里描述的互补观察结果是独特的，因为只有在反应的一个方向（即蔗糖裂解）而不是另一个方向（蔗糖合成）上才注意到这种可逆酶活性的升高。
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