Department of Horticulture and Landscape Architecture, Washington State University, 99164-6414, Pullman, WA, USA.
Theor Appl Genet. 1983 Apr;65(1):47-51. doi: 10.1007/BF00276261.
Kernels of wild type maize (Zea mays L.) and the mutants shrunken-1 and shrunken-2 developed as much as in vivo when excised at five days post-pollination and grown in culture using existing methods. Mature kernels from culture exhibited their expected phenotypes. Starch, sugar and enzyme levels of kernels grown in culture were similar to those known to occur in kernels of the same genotypes grown in vivo. Differences in percentage germination of kernels grown in vitro were similar to those of kernels grown in vivo.
野生型玉米（Zea mays L.）和突变体 shrinken-1 和 shrinken-2 的胚乳在授粉后 5 天离体培养时，其发育程度与活体相当，并且可以使用现有的方法进行培养。从培养中获得的成熟胚乳表现出其预期的表型。在培养中生长的胚乳的淀粉、糖和酶水平与已知在相同基因型的活体胚乳中存在的水平相似。在体外培养的胚乳的发芽百分比差异与在活体中生长的胚乳的发芽百分比差异相似。
