Auerswald E A, Schubert A, Dolinar M, Gürtler L, Deinhardt F
Abteilung für Klinische Chemie, Universität München, FRG.
Biomed Biochim Acta. 1991;50(4-6):697-700.
The (K15R M52E) aprotinin is a recombinant molecule with a broader inhibition spectrum against serine proteinases and a higher affinity towards certain proteinases as compared to native aprotinin. This aprotinin variant was produced in E. coli, isolated and purified to homogeneity. The inhibitor was further tested for its effectiveness to reduce human immunodeficiency virus type 1 (HIV-1) replication. Virus growth was followed by an ELISA which detects the amount of virus core protein p24. At a concentration of 50 microM, the recombinant (K15R M52E) aprotinin clearly reduced HIV-1 replication in H9 cells.
（K15R M52E）抑肽酶是一种重组分子，与天然抑肽酶相比，它对丝氨酸蛋白酶具有更广泛的抑制谱，对某些蛋白酶具有更高的亲和力。这种抑肽酶变体在大肠杆菌中产生，经过分离和纯化达到均一性。进一步测试了该抑制剂减少1型人类免疫缺陷病毒（HIV-1）复制的有效性。通过检测病毒核心蛋白p24量的ELISA法监测病毒生长。在50微摩尔的浓度下，重组（K15R M52E）抑肽酶明显降低了H9细胞中HIV-1的复制。
