影响鞭毛膜黏附缺失突变的连锁与衣藻 mt 交配型基因座。
Linkage of Mutations Affecting minus Flagellar Membrane Agglutinability to the mt Mating-Type Locus of Chlamydomonas.
机构信息
Department of Biology, Washington University, St. Louis, Missouri 63130.
出版信息
Genetics. 1981 Sep;99(1):41-7. doi: 10.1093/genetics/99.1.41.
Two independently isolated mutant strains, imp-10 and imp-12, were obtained by UV irradiation of wild-type mating-type minus (wt(-)). Each fails to agglutinate sexually with gametes of either mating type, but mating and zygote formation can be elicited by agglutinating either strain to wt(+) gametes by means of anti-flagellar antiserum. Tetrad analysis of the resultant zygotes shows that both imp-10 and imp-12 are very closely linked to mt(-), with no recombinants observed. Diploid strains constructed between imp-10 or imp-12 and wt(+) gametes are wt(-), that is, they agglutinate and fuse like normal minus cells. Tetrad analysis of triploids from imp-10 diploid x wt(+) haploid crosses shows that only imp-10 and wt(+) products are recovered. A model is proposed to account for these results.
两个独立分离的突变株 imp-10 和 imp-12，是通过对野生型交配型负（wt(-)）进行紫外线照射而获得的。每个突变株都不能与任何一种交配型的配子发生凝集，但通过用抗鞭毛抗血清将任一菌株凝集到 wt(+)配子上，可以引发交配和受精卵形成。对所得受精卵的四分体分析表明，imp-10 和 imp-12 都与 mt(-)紧密连锁，没有观察到重组子。在 imp-10 或 imp-12 与 wt(+)配子之间构建的二倍体菌株是 wt(-)，即它们像正常的负细胞一样凝集和融合。从 imp-10 二倍体 x wt(+)单倍体杂交的三倍体四分体分析表明，只有 imp-10 和 wt(+)产物被回收。提出了一个模型来解释这些结果。
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