Cell and Developmental Biology, The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Genetics. 1978 Jun;89(2):235-43. doi: 10.1093/genetics/89.2.235.
Mutant strains of Chlamydomonas reinhardi that have lost their ability to undergo sexual agglutination via their flagellar tips have been induced to undergo zygotic cell fusion and meiosis, using a flagellar-directed antiserum. Genetic analysis of antiserum-mediated crosses involving five nonagglutinating mt(+) mutant strains reveals the following: (1) None of the mutations is linked to the mt locus. (2) All of the mutations are "sex-limited," meaning that they can be carried and transmitted by, but not expressed in, mt(-) cells. (3) Four of the mutations (imp-2, imp-5, imp-6, imp-7) are either allelic or closely linked to one another, with imp-8 defining a second locus.
已诱导丧失通过鞭毛尖端进行有性凝集能力的莱茵衣藻突变株通过鞭毛定向抗血清进行合子细胞融合和减数分裂。涉及五个不凝集 mt(+)突变株的抗血清介导杂交的遗传分析显示:(1) 没有一个突变与 mt 基因座相连。(2) 所有的突变都是“性限制”的,这意味着它们可以被 mt(-)细胞携带和传递,但不能在 mt(-)细胞中表达。(3) 四个突变(imp-2、imp-5、imp-6、imp-7)要么是等位基因,要么彼此紧密连锁,imp-8 定义了第二个基因座。