Department of Biology, B-022, University of California, San Diego, La Jolla, CA 92093.
Genetics. 1981 Oct;99(2):183-96. doi: 10.1093/genetics/99.2.183.
Eight independently isolated mutants of Dictyostelium discoideum that differentiate exclusively into stalk cells make up one complementation group and carry single recessive mutations at the stalky locus, stkA, located on linkage group II. KY19, a previously described strain that differentiates into spores, but not stalk cells, was found to possess a recessive mutation defining the stalkless locus, stlA, located on linkage group VI. An analysis of the properties of these mutants, together with the phenotype of a haploid double mutant carrying stkA and stlA indicates that stlA results in poorly organized stalk tubes and incomplete stalk cell differentiation, while stkA causes all of the cells to differentiate into stalk cells, even when not enclosed in the stalk tube. The significance of these results is discussed in relation to current theories of pattern formation in D. discoideum.
八个独立分离的盘基网柄菌突变体，仅分化为柄细胞，构成一个互补群，并携带位于连锁群 II 上的柄状位点 stkA 的单一隐性突变。KY19 是以前描述的一种菌株，它分化为孢子，但不是柄细胞，被发现具有位于连锁群 VI 上的无柄位点 stlA 的隐性突变。对这些突变体的特性的分析，以及带有 stkA 和 stlA 的单倍体双突变体的表型分析表明，stlA 导致柄管组织不良和不完全的柄细胞分化，而 stkA 导致所有细胞分化为柄细胞，即使不在柄管中。这些结果的意义与盘基网柄菌形态发生的当前理论有关。
