Babol-Pokora Katarzyna, Prośniak Adam, Jacewicz Renata, Berent Jarosław
Katedry i Zakładu Medycyny Sadowej Uniwersytetu Medycznego w łodzi.
Arch Med Sadowej Kryminol. 2006 Oct-Dec;56(4):228-31.
SNP analysis is among the most contemporary method for personal identification in forensic genetics, especially in analyses of untypical cases and in studies of degraded DNA samples from crime scenes. This paper shows the results of Central Poland population studies carried out using minisequencing and SNP-pentaplex, which contains 5 biallelic loci rs2294067, rs2282160, rs2070764, rs2277216 and rs1063739. DNA fragments were amplified in one multiplex PCR reaction and SNPs were identified by the minisequencing method. The combined PD was 0.9895253125, which makes the pentaplex useful for forensic applications.
单核苷酸多态性(SNP)分析是法医遗传学中用于个人识别的最现代方法之一,特别是在非典型案例分析以及犯罪现场降解DNA样本的研究中。本文展示了使用微测序和包含5个双等位基因位点rs2294067、rs2282160、rs2070764、rs2277216和rs1063739的SNP五重检测法对波兰中部人群进行研究的结果。DNA片段在一次多重聚合酶链反应(PCR)中进行扩增,并通过微测序法鉴定SNP。累积个体识别率为0.9895253125,这使得该五重检测法适用于法医应用。