Janica Jerzy, Pepiński Witold, Skawrońska Małgorzata, Niemcunowicz-Janica Anna, Koc-Zurawska Ewa, Sołtyszewski Ireneusz
Zakładu Medycyny Sadowej AM w Białymstoku.
Arch Med Sadowej Kryminol. 2006 Oct-Dec;56(4):232-5.
The objective of the paper is to determine the allele frequencies for four X-chromosomal STRs determined in a population sample of 294 unrelated volunteers (191 males and 103 females) belonging to the Belarusian minority residing in northeastern Poland. A commercially available kit Mentype Argus X-UL (Biotype AG, Germany) was used to co-amplify X-STR loci: DXS8378, DXS7132, HPRTB and DXS7423. Electrophoresis and typing were performed by automated fluorescent detection in an ABI 310 Genetic Analyzer. The genotype distributions among the females conformed to HWE for all the analyzed loci. The evaluated quadruplex is a potential extension to a battery of autosomal systems in forensic applications, particularly in kinship analysis and in casework samples from sexual assaults.
本文的目的是确定在居住于波兰东北部的白俄罗斯少数民族的294名无血缘关系志愿者(191名男性和103名女性)的群体样本中测定的四个X染色体短串联重复序列(STR)的等位基因频率。使用市售的Mentype Argus X-UL试剂盒(德国Biotype AG公司)对X-STR基因座DXS8378、DXS7132、HPRTB和DXS7423进行共扩增。在ABI 310遗传分析仪中通过自动荧光检测进行电泳和分型。所有分析基因座的女性基因型分布均符合哈迪-温伯格平衡(HWE)。评估的四重检测体系是法医应用中一系列常染色体系统的潜在扩展,特别是在亲缘关系分析和性侵犯案件样本中。