Proks P, Hurnák O, Zachar J
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Gen Physiol Biophys. 1991 Dec;10(6):537-48.
Low-conductance chloride channel from skeletal muscle SR vesicles of the crayfish Astacus fluviatilis was incorporated into planar lipid bilayers and its basic characteristics were investigated. The channel has a relatively low unitary conductance of 26 pS in symmetrical 160 mmol/l choline-chloride. The dependence of the channel conductance on Cl- concentration shows saturating behavior with a maximum conductance of 37 pS and an ionic activity for half-maximum conductance Km = 75 mmol/l. The channel exhibits a complex kinetics with several modes of activity. Open state probability slightly decreases with the increasing absolute value of voltage. The channel activity does not appear to be dependent on the presence of Ca2+ ions. The channel is effectively inhibited by DIDS, a stilbene derivative. The permeability properties of the channel are similar to the specific behavior of the "double-barrelled" channel from Torpedo electroplax described by Miller and White (1984).
将小龙虾（Astacus fluviatilis）骨骼肌肌浆网囊泡中的低电导氯离子通道整合到平面脂质双分子层中，并对其基本特性进行了研究。该通道在对称的160 mmol/L氯化胆碱中具有相对较低的单通道电导，为26 pS。通道电导对Cl-浓度的依赖性表现出饱和行为，最大电导为37 pS，半最大电导的离子活性Km = 75 mmol/L。该通道表现出具有多种活动模式的复杂动力学。开放态概率随电压绝对值的增加而略有降低。通道活性似乎不依赖于Ca2+离子的存在。该通道被一种芪衍生物DIDS有效抑制。该通道的通透性特性类似于Miller和White（1984）描述的电鳐（Torpedo electroplax）“双桶”通道的特定行为。
