平面脂质双层系统中兔横小管Cl(-)通道的特性分析

Characterization of a Cl(-)-channel from rabbit transverse tubules in the planar lipid bilayer system.

作者信息

Hidaka J, Ide T, Kawasaki T, Taguchi T, Kasai M

机构信息

Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.

出版信息

Biochem Biophys Res Commun. 1993 Mar 31;191(3):977-82. doi: 10.1006/bbrc.1993.1313.

DOI:10.1006/bbrc.1993.1313

PMID:8385458

Abstract

A Cl(-)-channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl(-)-channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl(-)-channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl(-)-current from the cis-side to which the TT vesicles were added.

摘要

将横管（TT）的氯离子通道整合到人工平面脂质双分子层中，并对其特性进行了研究。发现该通道具有以下特性。（1）在胆碱 - 氯溶液中，单通道电导为40 pS。（2）通道的门控速率不依赖于膜电压。（3）该通道被二苯乙烯衍生物（DIDS和SITS）阻断，这两种物质是已知的电鳐电器官电压依赖性氯离子通道的抑制剂，且从膜的两侧均可阻断。（4）骨骼肌电压依赖性氯离子通道的抑制剂9 - 蒽甲酸（9 - AC）可抑制从添加了TT囊泡的顺式侧的氯离子电流。