González-Rioja R, Ferrer A, Arilla M C, Ibarrola I, Viguera A R, Andreu C, Martínez A, Asturias J A
Research & Development Department, Bial-Arístegui, Bilbao, Spain.
Clin Exp Allergy. 2007 Feb;37(2):243-50. doi: 10.1111/j.1365-2222.2007.02643.x.
Parietaria judaica pollen is one of the main causes of allergic diseases in the Mediterranean area and contains two major allergens, called Par j 1 and Par j 2.
To evaluate the diagnostic potential of natural and recombinant forms of Par j 1 and Par j 2 in comparison with standardized P. judaica pollen extract.
Thirty patients allergic to P. judaica pollen and 15 control patients were investigated. Skin prick tests and determination of specific IgE levels were performed with commercial P. judaica extract, natural Par j 1 and Par j 2, and recombinant forms of both allergens expressed in P. pastoris.
The whole group of patients with allergy to P. judaica had a positive skin test reaction to purified nPar j 1-Par j 2 and rPar j 2 at 5 microg/mL, and no false-positive reactions were detected. Natural and recombinant Par j 1 and Par j 2 showed no significantly different responses in skin tests compared with P. judaica extract. A high correlation was found between the serum-specific IgE levels to P. judaica extract vs. natural (R=0.996; P<0.001) and recombinant allergens (R=0.887 and 0.982 for rPar j 1 and rPar j 2, respectively; P<0.001). rPar j 2 displayed a 100% sensitivity and specificity among P. judaica-allergic patients.
In vivo and in vitro diagnosis of P. judaica pollen allergy could be simplified using rPar j 2. This protein showed comparable IgE response and skin prick reactivity with those produced by P. judaica pollen extract.
墙草花粉是地中海地区过敏性疾病的主要病因之一，包含两种主要过敏原，即Par j 1和Par j 2。
比较天然和重组形式的Par j 1及Par j 2与标准化墙草花粉提取物的诊断潜力。
对30例对墙草花粉过敏的患者和15例对照患者进行研究。采用市售墙草提取物、天然Par j 1和Par j 2以及在巴斯德毕赤酵母中表达的两种过敏原的重组形式进行皮肤点刺试验并测定特异性IgE水平。
对墙草过敏的患者组对5μg/mL的纯化天然Par j 1 - Par j 2和重组Par j 2皮肤试验反应呈阳性，未检测到假阳性反应。与墙草提取物相比，天然和重组的Par j 1及Par j 2在皮肤试验中的反应无显著差异。血清中对墙草提取物的特异性IgE水平与天然过敏原（R = 0.996；P < 0.001）及重组过敏原（重组Par j 1和重组Par j 2的R分别为0.887和0.982；P < 0.001）之间存在高度相关性。重组Par j 2在对墙草过敏的患者中显示出100%的敏感性和特异性。
使用重组Par j 2可简化墙草花粉过敏的体内和体外诊断。该蛋白与墙草花粉提取物产生的IgE反应和皮肤点刺反应性相当。
