Heindel N D, Zhao H R, Egolf R A, Chang C H, Schray K J, Emrich J G, McLaughlin J P, Woo D V
Department of Chemistry, Lehigh University, Bethelehem, Pennsylvania 18015.
Bioconjug Chem. 1991 Nov-Dec;2(6):427-30. doi: 10.1021/bc00012a008.
A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17-1A, or an Fab' derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.
