Ballough D U, Ballough G P, Strauss J A, Durkot M J, Anthony A
Department of Biology, Pennsylvania State University, University Park 16802.
Cell Biochem Funct. 1991 Oct;9(4):287-92. doi: 10.1002/cbf.290090411.
The present investigation was undertaken to examine possible noise-induced alterations in adrenal fasciculata cell (AFC) metabolism, and also to determine if the magnitude of these changes differs in male versus female rats. Wistar rats approximately 3 months old were exposed to intense noise for 60 min (100 dB, re 2 x 10(-5) N(m2)-1, 350-20,000 Hz); control rats were housed under identical conditions, at an ambient noise level of 40-60 dB. Adrenal fasciculata cells (AFC) from each animal were examined for noise-induced alterations in Feulgen-DNA reactivity (as an indicator of chromatin template activity) and Coomassie-total cell protein levels using scanning-integrating cytophotometry. The results provide evidence that intense noise elicited a marked AFC metabolic enhancement in both male and female rats; the degree of this enhancement was more pronounced in males. This disparity may be due to pre-existing differences in male versus female AFC enzymatic capability and subsequent responsiveness to noise-induced activation.
本研究旨在探讨噪声是否会引起肾上腺束状带细胞（AFC）代谢的改变，并确定这些变化在雄性和雌性大鼠中的程度是否存在差异。将约3个月大的Wistar大鼠暴露于高强度噪声60分钟（100分贝，基准为2×10⁻⁵牛顿/平方米⁻¹，350 - 20000赫兹）；对照大鼠饲养在相同条件下，环境噪声水平为40 - 60分贝。使用扫描积分细胞光度法检测每只动物的肾上腺束状带细胞（AFC）的噪声诱导的孚尔根-DNA反应性（作为染色质模板活性的指标）和考马斯亮蓝-总细胞蛋白水平的变化。结果表明，高强度噪声在雄性和雌性大鼠中均引起了显著的AFC代谢增强；这种增强在雄性中更为明显。这种差异可能是由于雄性和雌性AFC酶能力的预先存在的差异以及随后对噪声诱导激活的反应性不同所致。
