Hatta Mochammad, Smits Henk L
Department of Medical Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia.
Am J Trop Med Hyg. 2007 Jan;76(1):139-43.
A nested polymerase chain reaction (PCR) specific for Salmonella enterica serovar Typhi was used for the detection of the pathogen in blood, urine, and stool samples from 131 patients with clinical suspicion of typhoid fever. The sensitivity of blood culture, the PCRs with blood, urine, and feces, and the Widal test were 61.8%, 84.5%, 69.3%, 46.9%, and 39.0%, respectively. The sensitivity of the PCRs with blood (P < 0.001) and urine (P = 0.01) were significantly higher, and the sensitivity of the PCR with feces (P > 0.05) was similar to that of blood culture. Combined, the PCRs on urine and feces showed positive results for 16 (70%) of 23 typhoid patients with negative results with blood culture and PCR with blood. These results show that the PCR with blood is a sensitive method for the diagnosis of typhoid fever, and that the PCRs with urine and feces could be useful complementary tests.
采用针对伤寒沙门氏菌的巢式聚合酶链反应（PCR）检测131例临床疑似伤寒热患者血液、尿液和粪便样本中的病原体。血培养、血液PCR、尿液PCR、粪便PCR以及肥达试验的敏感性分别为61.8%、84.5%、69.3%、46.9%和39.0%。血液PCR（P < 0.001）和尿液PCR（P = 0.01）的敏感性显著更高，粪便PCR的敏感性（P > 0.05）与血培养相似。尿液和粪便PCR联合检测显示，23例血培养和血液PCR结果为阴性的伤寒患者中有16例（70%）呈阳性。这些结果表明，血液PCR是诊断伤寒热的一种敏感方法，尿液和粪便PCR可作为有用的补充检测方法。
