Joyner Priscilla H, Shreve Allison A, Spahr John, Fountain Andrea L, Sleeman Jonathan M
The Wildlife Center of Virginia, PO Box 1557, Waynesboro, Virginia 22980, USA.
J Wildl Dis. 2006 Oct;42(4):883-8. doi: 10.7589/0090-3558-42.4.883.
A free-ranging eastern box turtle (Terrapene carolina carolina) was referred to the Wildlife Center of Virginia with a three-month history of marked swelling of the right hind limb initially diagnosed as chromomycosis by histopathology. Hematology revealed severe anemia (9%), leukocytosis (12.8 cells x 10(3)/microl), heterophilia (6.14 cells x 10(3)/microl), and monocytosis (0.51 cells x 10(3)/microl). Gross necropsy revealed a firm, encapsulated 3 x 1 cm subcutaneous mass filled with dark brown-black, friable necrotic material of the distal right hind limb. Microscopically, the mass was characterized by a granulomatous inflammatory process with numerous multinucleated histiocytic giant cells. Fungal elements were present within necrotic centers and associated with multinucleated cells. Special stains revealed numerous phaeoid hyphae and yeast; Exophiala jeanselmei was isolated by routine mycologic culture. Phaeohyphomycosis was diagnosed based on the histologic appearance of the fungal elements within the mass and culture results. There was no histopathological evidence of systemic infection. This is the first report of phaeohyphomycosis caused by fungi of the genus Exophiala in free-living reptiles.
一只自由放养的东部箱龟（卡罗莱纳箱龟指名亚种，Terrapene carolina carolina）被送至弗吉尼亚野生动物中心，右后肢明显肿胀已有三个月病史，组织病理学最初诊断为着色芽生菌病。血液学检查显示严重贫血（9%）、白细胞增多（12.8×10³个/微升）、嗜异性粒细胞增多（6.14×10³个/微升）和单核细胞增多（0.51×10³个/微升）。大体尸检发现右后肢远端有一个坚实的、有包膜的3×1厘米皮下肿块，充满深棕黑色、易碎的坏死物质。显微镜下，肿块的特征是肉芽肿性炎症过程，有许多多核组织细胞性巨细胞。坏死中心有真菌成分，并与多核细胞相关。特殊染色显示有许多暗色菌丝和酵母；通过常规真菌培养分离出了甄氏外瓶霉。根据肿块内真菌成分的组织学表现和培养结果诊断为暗色丝孢霉病。没有全身感染的组织病理学证据。这是自由生活爬行动物中由外瓶霉属真菌引起的暗色丝孢霉病的首例报告。
