Rhine E D, Ní Chadhain S M, Zylstra G J, Young L Y
Biotechnology Center for Agriculture and the Environment, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
Biochem Biophys Res Commun. 2007 Mar 16;354(3):662-7. doi: 10.1016/j.bbrc.2007.01.004. Epub 2007 Jan 9.
Six novel bacterial strains were isolated from the environment which can oxidize arsenite [As(III)] to the less mobile form arsenate [As(V)] coupled to CO(2) fixation under either aerobic or denitrifying conditions. PCR primers were designed to the conserved molybdopterin domain of the large subunit of arsenite oxidase in order to identify the arsenite oxidase genes from these isolates. The amino acid sequences for the arsenite oxidases reported here were 72-74% identical to that of strain NT-26, the only previously reported autotrophic arsenite oxidizer. Indeed the autotrophic arsenite oxidase genes form a distinct phylogenetic group, separated from previously described heterotrophic arsenite oxidase genes, with the exception of the heterotroph Agrobacterium tumefaciens. The arsenite oxidase primers described here represent a powerful culture-independent tool to assess the diversity of arsenite oxidase genes in environmental bacteria.
从环境中分离出六种新型细菌菌株，它们在有氧或反硝化条件下，能将亚砷酸盐[As(III)]氧化为移动性较差的砷酸盐[As(V)]，并与二氧化碳固定相偶联。设计了针对亚砷酸氧化酶大亚基保守钼蝶呤结构域的PCR引物，以便从这些分离菌株中鉴定亚砷酸氧化酶基因。此处报道的亚砷酸氧化酶的氨基酸序列与菌株NT-26的氨基酸序列有72%-74%的同一性，NT-26是之前唯一报道的自养亚砷酸氧化菌。实际上，自养亚砷酸氧化酶基因形成了一个独特的系统发育组，与之前描述的异养亚砷酸氧化酶基因分开，除了异养菌根癌土壤杆菌。此处描述的亚砷酸氧化酶引物是一种强大的非培养工具，可用于评估环境细菌中亚砷酸氧化酶基因的多样性。
