以硫酸化β-环糊精作为手性选择剂，通过毛细管电泳对苯基缩水甘油酸酯进行对映体拆分。

Enantioselective separation of phenylglycidates by capillary electrophoresis employing sulfated beta-cyclodextrin as chiral selector.

作者信息

Wang Jianjun, Yuan Qipeng, Evans David G, Yang Liu, Zheng Guojun, Sun Wanru

机构信息

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):560-3. doi: 10.1016/j.jchromb.2006.12.017. Epub 2006 Dec 17.

DOI:10.1016/j.jchromb.2006.12.017

PMID:17257909

Abstract

A capillary electrophoresis (CE) method for the enantioseparation of phenylglycidates has been developed. Successful enantioseparation was achieved using sulfated beta-cyclodextrin as chiral selector in a phosphate buffer. The effects of varying pH, sulfated beta-cyclodextrin concentration and electrophoresis voltage were systematically investigated and the optimized separation conditions were thus obtained. When the migration time was set at the threshold value, it was found that the best enantioseparation was obtained at 10 kV with 3% (w/v) sulfated beta-cyclodextrin at pH 6.5. A range of substituted phenylglycidates were successfully separated using the method and the results shown to be superior to those obtained using gas chromatography (GC).

摘要

已开发出一种用于苯甘氨酸酯对映体分离的毛细管电泳（CE）方法。在磷酸盐缓冲液中使用硫酸化β-环糊精作为手性选择剂成功实现了对映体分离。系统研究了不同pH值、硫酸化β-环糊精浓度和电泳电压的影响，从而获得了优化的分离条件。当迁移时间设定为阈值时，发现在pH 6.5下，使用3%（w/v）硫酸化β-环糊精，在10 kV电压下可获得最佳对映体分离效果。使用该方法成功分离了一系列取代苯甘氨酸酯，结果表明优于使用气相色谱（GC）获得的结果。