LEDGF/p75干扰体外催化HIV-1 DNA完全位点整合的突触核蛋白复合物的形成：对病毒cDNA整合机制的启示。
LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration.
作者信息
Raghavendra Nidhanapati K, Engelman Alan
机构信息
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
出版信息
Virology. 2007 Mar 30;360(1):1-5. doi: 10.1016/j.virol.2006.12.022. Epub 2007 Jan 26.
Abstract
An integrase dimer can process and integrate a single HIV-1 DNA end in vitro, whereas a tetramer is required to integrate two ends. LEDGF/p75 can potently stimulate integrase activity, but its effects on half- versus full-site integration have not been investigated. Stimulation of half-site but inhibition of full-site integration is revealed here. LEDGF/p75 seems to interfere with integrase oligomerization, but does not inhibit the catalytic activity of pre-assembled complexes. We therefore speculate that LEDGF/p75 function is restricted to a point in the viral lifecycle that occurs after the formation of the preintegration synaptic complex, for example, as a chromatin-associated tethering factor.
摘要
整合酶二聚体在体外可处理并整合单个HIV-1 DNA末端，而整合两个末端则需要四聚体。LEDGF/p75可有效刺激整合酶活性，但其对半位点与全位点整合的影响尚未得到研究。本文揭示了其对半位点整合的刺激作用以及对全位点整合的抑制作用。LEDGF/p75似乎会干扰整合酶的寡聚化，但不抑制预组装复合物的催化活性。因此，我们推测LEDGF/p75的功能局限于病毒生命周期中在预整合突触复合物形成之后的某个阶段，例如作为一种与染色质相关的拴系因子。
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Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75.HIV-1整合酶相互作用蛋白LEDGF/p75的染色质结合结构域的鉴定与表征
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A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo.一种由核定位信号和两个AT钩基序组成的三方DNA结合元件，在体内介导LEDGF/p75与染色质的结合。
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