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纳滤和微滤膜生物反应器的污泥特性及基于PCR-DGGE的微生物多样性比较

Comparison of sludge characteristics and PCR-DGGE based microbial diversity of nanofiltration and microfiltration membrane bioreactors.

作者信息

Choi Jae-Hoon, Lee Sang Hyon, Fukushi Kensuke, Yamamoto Kazuo

机构信息

Department of Urban Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan.

出版信息

Chemosphere. 2007 Apr;67(8):1543-50. doi: 10.1016/j.chemosphere.2006.12.004. Epub 2007 Jan 26.

DOI:10.1016/j.chemosphere.2006.12.004
PMID:17258282
Abstract

The objective of this study was to compare the sludge characteristics and microbial community diversity between the submerged nanofiltration membrane bioreactor (NF MBR) and microfiltration membrane bioreactor (MF MBR) treating the same municipal wastewater. The influence of a higher concentration of organic matter and salt was investigated. The results of water qualities showed that the dissolved organic carbon (DOC), total phosphorus (T-P) and salt concentrations of the supernatant in the NF MBR were three, four and two times as high as those in the MF MBR, respectively. The specific oxygen uptake rate of the NF MBR (2.9+/-0.4 mg O(2)g(-1)MLSSh(-1)) was lower than that of the MF MBR (4.3+/-1.1 mg O(2)g(-1)MLSS h(-1)). Result of extractable extracellular polymeric substances showed that the NF MBR sludge had more protein and less polysaccharide compared to the MF MBR sludge, whereas specific amount of total organic carbon were the same in both MBRs. The median floc diameters of the NF MBR and the MF MBR were 72+/-12 microm and 59+/-12 microm, respectively, which could be attributable to the different polysaccharide concentrations between both MBR mixed liquor. A higher concentration of materials (DOC, T-P and salt) in the bioreactor, determined by the high rejection rate of the NF membrane, did not significantly affect the microbial diversity under similar operation conditions.

摘要

本研究的目的是比较处理相同城市污水的浸没式纳滤膜生物反应器(NF MBR)和微滤膜生物反应器(MF MBR)之间的污泥特性和微生物群落多样性。研究了较高浓度的有机物和盐的影响。水质结果表明,NF MBR中上清液的溶解有机碳(DOC)、总磷(T-P)和盐浓度分别是MF MBR中的三倍、四倍和两倍。NF MBR的比氧摄取率(2.9±0.4 mg O₂g⁻¹MLSSh⁻¹)低于MF MBR(4.3±1.1 mg O₂g⁻¹MLSS h⁻¹)。可提取细胞外聚合物的结果表明,与MF MBR污泥相比,NF MBR污泥含有更多的蛋白质和更少的多糖,而两个MBR中总有机碳的特定含量相同。NF MBR和MF MBR的絮体中位直径分别为72±12微米和59±12微米,这可能归因于两个MBR混合液中多糖浓度的差异。由NF膜的高截留率决定的生物反应器中较高浓度的物质(DOC、T-P和盐)在相似的运行条件下对微生物多样性没有显著影响。

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