Gowda P H, Subbaiah T K, Joshi S H
Division of Horticulture, GKVK, Bangalore, India.
Biotech Histochem. 1991;66(6):321-3. doi: 10.3109/10520299109109994.
The juice from the berries of Cocculus hirsutum was extracted and used for pollen fertility studies in various crops. Two stains were prepared: P.H. Ramanjini (PHR) stain and modified PHR stain. The modified PHR stain contains lactic acid and produces the best staining differentiation. The intensity of the staining was dependent on the thickness of the pollen cell walls, hence PHR stain is recommended for thick walled pollen grains and the modified PHR stain for pollen with relatively thin walls. The preparation of both the stains are very simple, quick and inexpensive.
提取了毛叶轮环藤浆果的汁液，并将其用于各种作物的花粉育性研究。制备了两种染色剂：PHR染色剂（P.H. Ramanjini染色剂）和改良PHR染色剂。改良PHR染色剂含有乳酸，能产生最佳的染色区分效果。染色强度取决于花粉细胞壁的厚度，因此推荐PHR染色剂用于厚壁花粉粒，改良PHR染色剂用于壁相对较薄的花粉。两种染色剂的制备都非常简单、快速且成本低廉。
