瘢痕疙瘩家系永生化B淋巴母细胞系的建立及其核型分析
[Establishment of immortalized B-lymphoblastoid cell lines of keloid pedigree and its karyotype analysis].
作者信息
Song Mei, Gao Jian-hua, Yan Xin, Liu Xiao-jun, Chen Yang
机构信息
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Dec;26(12):1760-2.
OBJECTIVE
To establish immortalized B-lymphoblastoid cell lines of keloid pedigree transformed with Epstein-Barr (EB) virus and conduct karyotype analysis of the cells.
METHODS
Immortalized B-lymphoblastoid cell lines were established by EB virus transformation of the peripheral blood B lymphocytes from the members of keloid pedigree. Karyotype analysis was performed for the cultured cells of passages 10, 20, 30, and 35 to evaluate their genetic stability.
RESULTS
Altogether 27 immortalized lymphoblastoid cell lines with stable chromosome were obtained successfully from the keloid pedigree. No chromosomal abnormalities were found in the cultured cells until passages 30 and 35, in which variation in chromosome number and structure are detected.
CONCLUSION
The cell lines of the keloid pedigree established in this study can be useful in future studies, and genetic analysis is conducted preferably with cells of early passages.
目的
建立经爱泼斯坦-巴尔（EB）病毒转化的瘢痕疙瘩家系永生化B淋巴细胞系，并对细胞进行核型分析。
方法
通过EB病毒转化瘢痕疙瘩家系成员的外周血B淋巴细胞建立永生化B淋巴细胞系。对第10、20、30和35代培养细胞进行核型分析，以评估其遗传稳定性。
结果
成功从瘢痕疙瘩家系中获得了27个染色体稳定的永生化淋巴母细胞系。直到第30和35代培养细胞中才检测到染色体数目和结构的变化，在此之前未发现染色体异常。
结论
本研究建立的瘢痕疙瘩家系细胞系可用于未来研究，且遗传分析最好使用早期传代的细胞。
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