[晚期正常妊娠和子痫前期妊娠妇女胎盘组织中肾上腺髓质素的差异表达]
[Differential expression of adrenomedullin in the placentas of women with normal and preeclamptic pregnancies in late term].
作者信息
Gao Yun-fei, Yu Yan-hong, Li Zhi-qin
机构信息
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Dec;26(12):1828-30.
OBJECTIVE
To investigate the differential expression of adrenomedullin (ADM) in the placentas of women with normal and preeclamptic pregnancies, and explore the importance of ADM and its signal pathway in the development of preeclampsia.
METHODS
Ten pregnant women complicated with preeclampsia during the late term(>or=35 wk) were selected for this study along with 7 normal control pregnant women (>or=39 wk). The total RNA was extracted and sections of fresh placental tissues were prepared, and ADM expressions at mRNA and protein levels in women with normal and preeclamptic pregnancies were determined by Northern blotting and immunohistochemistry, respectively.
RESULT AND CONCLUSION
At both mRNA and protein levels, the expression of ADM in the placentas of preeclamptic women was significantly reduced obviously as compared with that of the normal control (P<0.05), suggesting that ADM expression reduction in preeclamptic placenta might be associated with the development of preeclampsia.
目的
研究肾上腺髓质素（ADM）在正常妊娠和子痫前期妊娠妇女胎盘组织中的差异表达，探讨ADM及其信号通路在子痫前期发病中的作用。
方法
选取10例晚期（≥35周）子痫前期孕妇及7例正常晚期（≥39周）孕妇作为研究对象。提取胎盘组织总RNA并制备新鲜胎盘组织切片，分别采用Northern印迹法和免疫组织化学法检测正常妊娠和子痫前期妊娠妇女胎盘组织中ADM mRNA和蛋白的表达水平。
结果与结论
子痫前期妊娠妇女胎盘组织中ADM mRNA和蛋白表达水平均明显低于正常对照组（P<0.05），提示子痫前期胎盘组织中ADM表达降低可能与子痫前期的发病有关。
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