Zhang Yong, Liang Jia-Li, Wang Sheng, Wang Hui, Zheng Xiao-Zhou, Yang Zhe, Zhang Bo
Department of Cardiovascular Surgery, Ji'nan Command General Hospital of PLA, Jinan 250031, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Jan;27(1):43-5, 48.
To test the effect of autologus marrow stromal cells (MSCs) transplantation combined with application of granulocyte colony stimulating factor (G-CSF) on the function of ischemic hearts in vivo.
Acute myocardial infarction was induced in rabbits by occlusion of the left anterior descending artery, and autologous MSCs labeled with BrdU in vitro was introduced in the infarct area of the same donor rabbit. G-CSF was administered by subcutaneous injection for 5 consecutive days. Four weeks later, the transplanted MSCs were detected by laser scanning confocal microscopy and the cardiac function of the rabbits was examined by echocardiogram and multichannel physiological recorder. The myocardial infarct size was measured on the mid-transverse sections stained with Masson's trichrome.
Four weeks after transplantation, the MSCs were found to undergo myogenic differentiation with expressions of alpha-sarcomeric actin and connexin 43 in the intercalated disk. MSC transplantation in combination with G-CSF administration improved the left ventricular contractility and markedly reduced myocardial infarct size as compared with cell transplantation without G-CSF.
Application of G-CSF following autologous MSC transplantation may represent a promising therapeutic strategy for ischemic heart disease.
在体内试验自体骨髓基质细胞（MSCs）移植联合应用粒细胞集落刺激因子（G-CSF）对缺血心脏功能的影响。
通过结扎左冠状动脉前降支诱导家兔急性心肌梗死，将体外经5-溴脱氧尿嘧啶核苷（BrdU）标记的自体MSCs注入同一供体家兔的梗死区域。连续5天皮下注射G-CSF。4周后，采用激光扫描共聚焦显微镜检测移植的MSCs，并用超声心动图和多通道生理记录仪检查家兔的心功能。在经马松三色染色的心脏横断面上测量心肌梗死面积。
移植4周后，发现MSCs发生肌源性分化，闰盘中有α-肌动蛋白和连接蛋白43表达。与未使用G-CSF的细胞移植相比，MSCs移植联合G-CSF给药改善了左心室收缩功能，并显著减小了心肌梗死面积。
自体MSCs移植后应用G-CSF可能是一种有前景的缺血性心脏病治疗策略。
