[可生物降解聚乳酸-甲壳质接骨板体外骨接合术的细胞毒性评估]
[Cytotoxicity evaluation of biodegradable polylactic acid-chitin plates in osteosynthesis in vitro].
作者信息
Li Yi, Li Shao-Ping
机构信息
Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Jan;27(1):65-8.
OBJECTIVE
To evaluate the cytotoxicity of polylactic acid-chitin plates in vitro.
METHODS
The cytotoxicity of polylactic acid-chitin plates was evaluated with MTT assay and direct contact assay using the cell line L929 in vitro. L929 cells were cultured with different concentrations of the extracts of polylactic acid-chitin plates (0, 50% and 100%, V/V) in vitro, and MTT assay was performed to evaluate the cell proliferation at 2, 4 and 7 days. The morphologic changes of the cells were observed by inverted microscope and scanning electron microscope.
RESULTS
MTT assay revealed that the extracts of polylactic acid-chitin plates enhanced the cell proliferation (P<0.01), but not in a dose-dependent manner (P>0.05). In the direct contact assay, no side effects were found in regard to the cell growth, proliferation and attachment to the polylactic acid-chitin plates as compared with the negative control. The cytotoxicity grade of polylactic acid-chitin plates was 0 or 1, suggesting that the material was almost free of cytotoxicity.
CONCLUSION
The polylactic acid-chitin plate possesses ideal cellular compatibility, and therefore has great potential for clinical use as an internal bone fixation material.
目的
体外评价聚乳酸 - 甲壳素板的细胞毒性。
方法
采用MTT法和直接接触法，以L929细胞系体外评价聚乳酸 - 甲壳素板的细胞毒性。体外将L929细胞与不同浓度的聚乳酸 - 甲壳素板提取物（0、50%和100%，V/V）共培养，分别于2、4和7天进行MTT法检测以评估细胞增殖情况。通过倒置显微镜和扫描电子显微镜观察细胞的形态学变化。
结果
MTT法显示，聚乳酸 - 甲壳素板提取物可促进细胞增殖（P<0.01），但并非呈剂量依赖性（P>0.05）。在直接接触法中，与阴性对照相比，未发现聚乳酸 - 甲壳素板对细胞生长、增殖及贴附有任何副作用。聚乳酸 - 甲壳素板的细胞毒性分级为0或1级，表明该材料几乎无细胞毒性。
结论
聚乳酸 - 甲壳素板具有理想的细胞相容性，因此作为一种内固定材料在临床上具有巨大的应用潜力。
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