Reeser Ryan J, Medler Robert T, Billington Stephen J, Jost B Helen, Joens Lynn A
Department of Veterinary Science and Microbiology, University of Arizona, 1117 East Lowell Street, Tucson, AZ 85721, USA.
Appl Environ Microbiol. 2007 Mar;73(6):1908-13. doi: 10.1128/AEM.00740-06. Epub 2007 Jan 26.
Campylobacter jejuni is a major cause of human diarrheal disease in many industrialized countries and is a source of public health and economic burden. C. jejuni, present as normal flora in the intestinal tract of commercial broiler chickens and other livestock, is probably the main source of human infections. The presence of C. jejuni in biofilms found in animal production watering systems may play a role in the colonization of these animals. We have determined that C. jejuni can form biofilms on a variety of abiotic surfaces commonly used in watering systems, such as acrylonitrile butadiene styrene and polyvinyl chloride plastics. Furthermore, C. jejuni biofilm formation was inhibited by growth in nutrient-rich media or high osmolarity, and thermophilic and microaerophilic conditions enhanced biofilm formation. Thus, nutritional and environmental conditions affect the formation of C. jejuni biofilms. Both flagella and quorum sensing appear to be required for maximal biofilm formation, as C. jejuni flaAB and luxS mutants were significantly reduced in their ability to form biofilms compared to the wild-type strain.
空肠弯曲菌是许多工业化国家人类腹泻疾病的主要病因，也是公共卫生和经济负担的一个来源。空肠弯曲菌作为商品肉鸡和其他家畜肠道中的正常菌群存在，可能是人类感染的主要来源。在动物生产供水系统中发现的生物膜中存在空肠弯曲菌，可能在这些动物的定殖中起作用。我们已经确定，空肠弯曲菌可以在供水系统中常用的各种非生物表面上形成生物膜，如丙烯腈丁二烯苯乙烯和聚氯乙烯塑料。此外，在营养丰富的培养基中生长或高渗透压会抑制空肠弯曲菌生物膜的形成，而嗜热和微需氧条件会增强生物膜的形成。因此，营养和环境条件会影响空肠弯曲菌生物膜的形成。鞭毛和群体感应似乎都是最大程度形成生物膜所必需的，因为与野生型菌株相比，空肠弯曲菌flaAB和luxS突变体形成生物膜的能力显著降低。
