Department of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, and Department of Physical and Colloid Chemistry, Wageningen Agricultural University, Dreijenplein 6, 6703 HB Wageningen, The Netherlands.
Appl Environ Microbiol. 1993 Oct;59(10):3255-65. doi: 10.1128/aem.59.10.3255-3265.1993.
The deposition of various pseudomonads and coryneform bacteria with different hydrophobicities (water contact angles) and negative cell surface charges on negatively charged Teflon and glass surfaces was investigated. The levels of deposition varied between 5.0 x 10 and 1.6 x 10 cells cm and between 5.0 x 10 and 3.6 x 10 cells cm for dynamic column and static batch systems, respectively, indicating that there was a wide variation in physicochemical interactions. Batch and column results were compared in order to better distinguish between hydrodynamic and other system-dependent influences and method-independent physicochemical interactions. Despite the shorter suspension-solid contact time in columns (1 h) than in batch systems (4 h), the level of deposition (expressed as the number of cells that adhered) divided by the applied ambient cell concentration was 4.12 +/- 1.63 times higher in columns than in batch sytems for 15 of 22 strain-surface combinations studied. This demonstrates that transport of microbial particles from bulk liquid to surfaces is more efficient in dynamic columns (transport dominated by convection and diffusion) than in static batch systems (transport by diffusion only). The relative constancy of this ratio for the 15 combinations shows that physicochemical interactions affect adhesion similarly in the two systems. The deviating deposition behavior of the other seven strain-surface combinations could be attributed to method-dependent effects resulting from specific cell characteristics (e.g., to the presence of capsular polymers, to an ability to aggregate, to large cell sizes, or to a tendency to desorb after passage through an air-liquid interface).
研究了不同疏水性（水接触角）和带负电荷的假单胞菌和棒状杆菌的不同菌株在带负电荷的特氟龙和玻璃表面上的沉积情况。在动态柱和静态分批系统中，沉积水平分别在 5.0 x 10 和 1.6 x 10 细胞 cm 和 5.0 x 10 和 3.6 x 10 细胞 cm 之间变化，表明存在广泛的物理化学相互作用。比较了分批和柱系统的结果，以便更好地区分水动力和其他系统相关影响与方法独立的物理化学相互作用。尽管柱系统（1 小时）中的悬浮-固体接触时间比分批系统（4 小时）短，但在 22 种菌株-表面组合中有 15 种的沉积水平（表示为附着的细胞数量）除以施加的环境细胞浓度，柱系统比分批系统高 4.12 +/- 1.63 倍。这表明微生物颗粒从主体液体到表面的传输在动态柱系统（传输主要由对流和扩散控制）中比在静态分批系统（仅通过扩散传输）中更有效。对于这 15 种组合，该比率的相对稳定性表明物理化学相互作用在两种系统中以相似的方式影响附着。其余七种菌株-表面组合的不同沉积行为可归因于特定细胞特性导致的方法依赖性影响（例如，存在荚膜聚合物、聚集能力、大细胞尺寸或通过气液界面传递后的解吸趋势）。
