Guo X, Wang A, Li X, Guo Z, Zhang Y
PUMC Hospital, CAMS, Beijing.
Chin Med Sci J. 1991 Dec;6(4):212-6.
Three hybridoma cell lines producing monoclonal antibodies (McAb) against recombinant human granulocyte colony-stimulating factor (rhG-CSF) have been established by fusing mouse myeloma cell line SP2/0 cells with spleen cells from a BALB/c mouse immunized with rhG-CSF. Ascites was obtained from BALB/c mice by injecting the hybridoma cells intraperitoneally. Three McAbs were purified by the caprylic acid-ammonium sulfate method. For each, the IgG subclass, valence and activity, molecular weight, specificity and affinity were determined. The applications of McAbs against rhG-CSF in the clinic and laboratory are discussed.
通过将小鼠骨髓瘤细胞系SP2/0细胞与用重组人粒细胞集落刺激因子（rhG-CSF）免疫的BALB/c小鼠的脾细胞融合，建立了三种产生抗重组人粒细胞集落刺激因子单克隆抗体（McAb）的杂交瘤细胞系。通过腹腔注射杂交瘤细胞从BALB/c小鼠获得腹水。用辛酸-硫酸铵法纯化三种单克隆抗体。分别测定了每种单克隆抗体的IgG亚类、效价和活性、分子量、特异性和亲和力。讨论了抗rhG-CSF单克隆抗体在临床和实验室中的应用。
