Price M R, Briggs S, Scanlon M J, Tendler S J, Sibley P E, Hand C W
Cancer Research Campaign Laboratories, University of Nottingham.
Dis Markers. 1991 May-Aug;9(3-4):205-12.
Serum of breast cancer patients contains high molecular weight, mucin-like glycoproteins which are held to be differentiation markers for certain types of normal epithelia, in particular mammary epithelium. These components have primarily been identified using monoclonal antibodies raised against human milk fat globule membranes, tumour extracts or purified mucins. Even so, many of the antibodies produced react with a discrete region of the mucin protein core involving the hydrophilic turn domain APDTRPAP. The present investigation using the anti-urinary mucin antibody, C595, illustrates both the clinical potential of the mucin antigens in breast cancer studies as well as the exquisite specificity of immune recognition of a complex polymorphic glycoprotein at the level of the individual amino acids.
乳腺癌患者的血清中含有高分子量的、黏蛋白样糖蛋白，这些糖蛋白被认为是某些类型正常上皮细胞，尤其是乳腺上皮细胞的分化标志物。这些成分主要是通过使用针对人乳脂肪球膜、肿瘤提取物或纯化黏蛋白产生的单克隆抗体来鉴定的。即便如此，所产生的许多抗体与黏蛋白蛋白核心的一个离散区域发生反应，该区域涉及亲水性转折结构域APDTRPAP。本研究使用抗尿黏蛋白抗体C595，既展示了黏蛋白抗原在乳腺癌研究中的临床潜力，也展示了在单个氨基酸水平上对复杂多态性糖蛋白进行免疫识别的高度特异性。
