Niven D F, Collins P A, Knowles C J
J Gen Microbiol. 1975 Oct;90(2):271-85. doi: 10.1099/00221287-90-2-271.
The particulate fraction of disrupted Chromobacterium violaceum grown under cyanide-evolving conditions was unable to oxidize ascorbate plus N,N,N',N'-tetra-methyl-p-phenylenediamine (TMPD), but oxidized NADH and succinate by a linear respiratory pathway which was very resistant to inhibition by cyanide. When the bacteria were grown under conditions where little cyanide evolution occurred, particulate fractions developed the ability to oxidize ascorbate-TMPD by a pathway highly sensitive to cyanide inhibition; respiratory activity with NADH and succinate proceeded via both the cyanide-sensitive and-resistant pathways. Studies with respiratory inhibitors, and the cytochrome compositions of the fractions derived from cultures grown under both conditions, are presented. A soluble, carbon monoxide-binding cytochrome c was found, and this appears similar to those found recently in Beneckea natiegens, methylotrophic bacteria and the marine pseudomonad B16.
在产氰条件下培养的破碎的紫色色杆菌的颗粒部分不能氧化抗坏血酸加N，N，N'，N'-四甲基对苯二胺（TMPD），但通过对氰化物抑制具有很强抗性的线性呼吸途径氧化NADH和琥珀酸。当细菌在几乎不产氰的条件下培养时，颗粒部分通过对氰化物抑制高度敏感的途径获得了氧化抗坏血酸-TMPD的能力；NADH和琥珀酸的呼吸活性通过氰化物敏感和抗性途径进行。本文介绍了使用呼吸抑制剂的研究以及在两种条件下培养的培养物中获得的部分的细胞色素组成。发现了一种可溶性的、能结合一氧化碳的细胞色素c，这似乎与最近在嗜盐贝内克氏菌、甲基营养细菌和海洋假单胞菌B16中发现的细胞色素c相似。
