Popadich I A, Mirovich A I, Martynenko N S, Petrova O T
Prikl Biokhim Mikrobiol. 1975 Mar-Apr;11(2):303-6.
The paper describes studies of heat treatment of the preparations amylorizin G10x, amylosubtilin G10x and glucoendomycopsin G15 and its effect on the relationshop between the rate of the enzymic reaction and the preparation concentration, optimal pH and temperatures, and the EDTA influence on the activity of heated preparations. After heating the preparations showed an elevation of optimal temperatures of their action and a shift of optimal pH to the alkaline region. Primary heating of the preparations resulted in an increase of the rate of the enzymic reaction. Heated preparations were less susceptible to the effect of the inhibitor that nonheated ones.
该论文描述了对制剂淀粉糖化酶G10x、淀粉枯草杆菌蛋白酶G10x和葡糖内霉蛋白酶G15的热处理研究,及其对酶促反应速率与制剂浓度、最佳pH值和温度之间关系的影响,以及EDTA对加热制剂活性的影响。加热后,制剂的作用最佳温度升高,最佳pH值向碱性区域偏移。制剂的初次加热导致酶促反应速率增加。与未加热的制剂相比,加热后的制剂对抑制剂的作用更不敏感。