El'chits S V, Ianishpol'skiĭ V V, Abaimova M B, Zatorskaia L A
Ukr Biokhim Zh (1978). 1979 Jul-Aug;51(4):374-7.
The article deals with the process of immobilizing the enzymic preparation amylosubtilin G10x by the carriers activated with 2,4-toluylenediisocyanate and cyanurchloride which were obtained on the basis of silanized sylochrome. The 2,4-toluylenediisocyanate activated carrier is shown to be high-effective in immobilization. The activity of the preparations immobilized by the carrier activated with 2,4-toluylenediisocyatate is 75 units per 1 g of carrier; for the preparation immobilized on the carrier activated with cyanurchloride it is equal to 2.9 u/g. The specific activity is 3.1 and 0.9 u/mg, respectively. Immobilization on the tested carriers decreases the enzymic preparation thermostability.
本文研究了用基于硅烷化硅铝酸盐获得的2,4-甲苯二异氰酸酯和三聚氯氰活化的载体固定化酶制剂淀粉芽孢杆菌蛋白酶G10x的过程。结果表明,2,4-甲苯二异氰酸酯活化的载体在固定化方面具有高效性。用2,4-甲苯二异氰酸酯活化的载体固定化的制剂活性为每1克载体75单位;用三聚氯氰活化的载体固定化的制剂活性为2.9单位/克。比活性分别为3.1和0.9单位/毫克。在测试的载体上进行固定化会降低酶制剂的热稳定性。