一种使用铈和3,3'-二氨基联苯胺在凝胶和印迹上检测产H2O2氧化酶活性的新染色方法。
A new staining method for the detection of activities of H2O2-producing oxidases on gels and blots using cerium and 3,3'-diaminobenzidine.
作者信息
Seitz J, Keppler C, Fahimi H D, Völkl A
机构信息
Department of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
出版信息
Electrophoresis. 1991 Dec;12(12):1051-5. doi: 10.1002/elps.1150121210.
Cerium chloride (CeCl3) was used to trap the hydrogen peroxide generated by several oxidases on native gels and blots. The pale yellow color of cerium perhydroxide formed is converted to a brown-black precipitate by the subsequent reaction with 3,3'-diaminobenzidine. The suitability of this method for the detection of the activity of several oxidases on gels and on blots under nondenaturing conditions, employing different electrophoretic systems and resolving techniques, is demonstrated. Moreover, this method has proven to be highly suitable for the assessment of the substrate and stereospecificity of oxidases, the determination of their molecular weights, and the isoelectric points of isoforms.
氯化铈（CeCl3）用于捕获天然凝胶和印迹上几种氧化酶产生的过氧化氢。形成的过氢氧化铈的浅黄色通过随后与3,3'-二氨基联苯胺的反应转化为棕黑色沉淀。证明了该方法适用于在非变性条件下，采用不同的电泳系统和分离技术，检测凝胶和印迹上几种氧化酶的活性。此外，该方法已被证明非常适合评估氧化酶的底物和立体特异性、测定其分子量以及同工型的等电点。
Zotero 插件