van Goor H, Gerrits P O, Hardonk M J
Department of Pathology, University of Groningen, The Netherlands.
J Histochem Cytochem. 1989 Mar;37(3):399-403. doi: 10.1177/37.3.2465338.
We describe a new method for light microscopic demonstration of alkaline phosphatase (ALP) activity in plastic-embedded sections. Rat tissues were fixed in acetone (-20 degrees C), infiltrated in glycol methacrylate (GMA), and embedded at 0 degrees C. Sections were cut at 1 and 2 microns, dried at room temperature, and incubated in the conventional Gomori medium. Cerium chloride was used to convert calcium phosphate into cerium phosphate, which was subsequently converted into cerium perhydroxide. The slight yellow precipitate of cerium perhydroxide was amplified using 3,3'-diaminobenzidine tetrahydrochloride (DAB). For comparison, tissue sections were processed according to the calcium-cobalt method. The method described combines exact localization of ALP activity with optimal preservation of tissue morphology.
我们描述了一种在塑料包埋切片中进行碱性磷酸酶（ALP）活性光镜显示的新方法。将大鼠组织在丙酮（-20℃）中固定，用甲基丙烯酸乙二醇酯（GMA）渗透，并在0℃下包埋。切片厚度为1和2微米，在室温下干燥，然后在传统的Gomori培养基中孵育。使用氯化铈将磷酸钙转化为磷酸铈，随后将其转化为过氢氧化铈。用过二盐酸3,3'-二氨基联苯胺（DAB）放大过氢氧化铈的浅黄色沉淀。为作比较，组织切片按照钙钴法进行处理。所描述的方法将ALP活性的准确定位与组织形态的最佳保存相结合。
